Southern blotting - This lecture explains about the southern blotting procedure and technique to identify specific DNA fragment from a mixture of DNA molecules separated by the Agarose gel electrophoresis. Southern blotting step by step explanation is provided to understand the procedure of the DNA separation and identification. Southern blotting application is also explained in this video.
Southern blotting consists of three different stages -
1. First is the isolation of DNA from the target cell.
2. Then the treatment of restriction digestion of the DNA to generate DNA fragments
3. Then the separation of the DNA fragments using Agarose gel electrophoresis
4. Transfer of the Agarose gel DNA bands to a nitrocellulose Membrane filter. This filter paper after the DNA transfer is known as southern blot.
5. Then the use of complementary DNA probe to bind with the target DNA that will indicate the presence of target sequence by radioactivity or colorize trick expression. Southern blotting was invented by EM Southern on 1975 at it was named after him to identify DNA using blotting technique.
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